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Optimization of carbon and nitrogen sources and growth factors for the production of an aquaculture probiotic ( Pseudomonas MCCB 103) using response surface methodology
Author(s) -
Preetha R.,
Jayaprakash N.S.,
Philip R.,
Bright Singh I.S.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.03149.x
Subject(s) - food science , response surface methodology , biomass (ecology) , central composite design , mannitol , glycerol , chemistry , urea , nitrogen , bioprocess , factorial experiment , biology , biochemistry , chromatography , organic chemistry , agronomy , paleontology , statistics , mathematics
Aim: To develop a new medium for enhanced production of biomass of an aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine compound, pyocyanin. Methods and Results: Carbon and nitrogen sources and growth factors, such as amino acids and vitamins, were screened initially in a mineral medium for the biomass and antagonistic compound of Pseudomonas MCCB 103. The selected ingredients were further optimized using a full‐factorial central composite design of the response surface methodology. The medium optimized as per the model for biomass contained mannitol (20 g l −1 ), glycerol (20 g l −1 ), sodium chloride (5 g l −1 ), urea (3·3 g l −1 ) and mineral salts solution (20 ml l −1 ), and the one optimized for the antagonistic compound contained mannitol (2 g l −1 ), glycerol (20 g l −1 ), sodium chloride (5·1 g l −1 ), urea (3·6 g l −1 ) and mineral salts solution (20 ml l −1 ). Subsequently, the model was validated experimentally with a biomass increase by 19% and fivefold increase of the antagonistic compound. Conclusion: Significant increase in the biomass and antagonistic compound production could be obtained in the new media. Significance and Impact of the Study: Media formulation and optimization are the primary steps involved in bioprocess technology, an attempt not made so far in the production of aquaculture probiotics.