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Effect of ovotransferrin, protamine sulfate and EDTA combination on biofilm formation by catheter‐associated bacteria
Author(s) -
Yakandawala N.,
Gawande P.V.,
LoVetri K.,
Madhyastha S.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.03129.x
Subject(s) - biofilm , protamine sulfate , enterococcus faecalis , proteus mirabilis , microbiology and biotechnology , ethylenediaminetetraacetic acid , staphylococcus epidermidis , ovotransferrin , pseudomonas aeruginosa , chemistry , bacteria , klebsiella pneumoniae , escherichia coli , staphylococcus aureus , protamine , biology , biochemistry , transferrin , chelation , heparin , genetics , organic chemistry , gene
Aims: To determine the effect of a composition comprising ovotransferrin (OT), protamine sulfate (PS) and ethylenediaminetetraacetic acid (EDTA) on biofilm formation by catheter‐associated bacteria. Methods and Results: The in vitro activity of OT, PS and EDTA alone and in combinations against biofilm formation by Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa , Proteus mirabilis , Enterococcus faecalis and Staphylococcus epidermidis was investigated. All the three compounds either alone or in combinations failed to inhibit the growth completely at the concentrations tested. However, the subinhibitory concentrations of three compounds in a composition showed synergistic inhibitory effect on biofilm formation by K. pneumoniae , Ps. aeruginosa and S. epidermidis . Furthermore, 79–95% reduction in Ps. aeruginosa and S. epidermidis biofilm formation was observed in a clear vinyl urinary catheter treated with the composition. Conclusion: The subinhibitory concentrations of OT, PS and EDTA in a composition were effective in reducing biofilm formation by catheter‐associated bacteria. Significance and Impact of the Study: This study shows that a synergistic composition‐comprising non‐antibiotic generally regarded as safe (GRAS) compounds such as OT, PS and EDTA may be used in the prevention of catheter‐related infections.