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Isolation and identification of a novel valienamine‐producing bacterium
Author(s) -
Wang Y.S.,
Zheng Y.G.,
Shen Y.C.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.03114.x
Subject(s) - bacteria , strain (injury) , biology , stenotrophomonas , stenotrophomonas maltophilia , bacterial strain , fermentation , 16s ribosomal rna , microbiology and biotechnology , biochemistry , genetics , pseudomonas aeruginosa , anatomy
Aims: To isolate, identify and assess valienamine production by a soil bacterial isolate from a wheat field in Hangzhou, China. Methods and Results: A validamycin A‐degrading bacterial strain, numbered ZJB‐041, was isolated and identified as Stenotrophomonas maltophilia , based on morphology, physiological tests, ATB system (ID32 GN), and 16S rDNA analysis. The strain was capable of producing valienamine by decomposing validamycin A. After fermentation in shaking flasks at 30°C for 7 days, 96·0% of 34·49 mmol l −1 of validamycin A was degraded and 2·65 mmol l −1 of valienamine was obtained. The resting cells of this strain also produced valienamine by degrading validamycin A. After 72 h of incubation in 0·2 mol l −1 of phosphate buffer (pH 7·5), 90·2% of 17·16 mmol l −1 of validamycin A was degraded, and 1·77 mmol l −1 of valienamine was obtained. Conclusions: Our data suggested that S. maltophilia ZJB‐041, a bacterial isolate, has the potential for validamycin A degradation and valienamine production. Significance and Impact of the Study: The validamycin A‐degrading bacterium could potentially be utilized in the disposal of validamycin residues and in the production of valienamine.