Premium
The tomato pathogen Clavibacter michiganensis ssp. michiganensis : producer of several antimicrobial substances
Author(s) -
Holtsmark I.,
Mantzilas D.,
Eijsink V.G.H.,
Brurberg M.B.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.03091.x
Subject(s) - clavibacter michiganensis , antimicrobial , microbiology and biotechnology , pathogen , bacteriocin , lantibiotics , biology , antimicrobial peptides , bacteria , chemistry , genetics
Aim: To purify and analyse antimicrobial substances produced by the tomato pathogen Clavibacter michiganensis ssp. michiganensis ( Cmm ), with potential application in control of Clavibacter michiganensis ssp. sepedonicus ( Cms ), the causal agent of bacterial ring rot of potato. Methods and Results: After selection of a suitable producer and indicator strain, antimicrobial compounds were isolated using chromatographic techniques. The resulting preparations were analysed with respect to heat and protease sensitivity, amino acid composition, amino acid sequence and mass. Using this procedure we discovered one post‐translationally modified 2145 Da peptide bacteriocin, one 14 kDa antimicrobial protein as well as low molecular weight (<1000 Da) antimicrobial compounds, putatively belonging to the tunicamycin family. Conclusions: Clavibacter michiganensis ssp. michiganensis produces various antibacterial substances that are active against Cms . Significance and Impact of the Study: This study describes the first attempt to characterize antimicrobial substances from Cmm at the molecular level. This is an important step towards investigation of the possible use of these compounds to control the potato ring rot pathogen.