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Production of enterolysin A by rumen Enterococcus faecalis strain and occurrence of enl A homologues among ruminal Gram‐positive cocci
Author(s) -
Nigutova K.,
Morovsky M.,
Pristas P.,
Teather R. M.,
Holo H.,
Javorsky P.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.03068.x
Subject(s) - bacteriocin , enterococcus faecalis , microbiology and biotechnology , structural gene , rumen , biology , bacteria , gel electrophoresis , streptococcaceae , escherichia coli , biochemistry , gene , fermentation , genetics , antimicrobial , antibiotics
Aims: Purification and partial characterization of an extracellular bacteriocin produced by the ruminal isolate Enterococcus faecalis II/1 and determine the frequency of occurrence of enterolysin A structural gene within the ruminal cocci. Methods and Results: Bacteriocin produced by E. faecalis II/1 was purified to homogeneity. Purified bacteriocin exhibited a single band on sodium dodecylsulphate polyacrylamide gel electrophoresis with an apparent molecular weight of about 35 kDa. The amino acid sequence of the first 30 amino acids of purified bacteriocin was identical with the enterolysin A sequence. The DNA sequence of the nearly complete E. faecalis II/1 bacteriocin structural gene was identical to the enterolysin A gene sequence, confirming that this bacteriocin is identical to enterolysin A, a cell wall‐degrading bacteriocin from E. faecalis LMG 2333. Enterolysin A structural genes were detected in approximately one‐sixth of the Gram‐positive ruminal cocci examined by PCR using primers targeting the enterolysin A structural gene. Conclusions: Bacteriocin produced by E. faecalis II/1 is identical to enterolysin A. Enterolysin A structural gene homologues are frequently encountered in rumen enterococcal and streptococcal bacterial strains. Significance and Impact of the study: This is the first evidence of a large heat‐labile bacteriocin produced by rumen E. faecalis strain, enlarging the number and types of known anti‐bacterial proteins produced by rumen bacteria.