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Effect of high hydrostatic pressure on four genotypes of F‐specific RNA bacteriophages
Author(s) -
Guan D.,
Joerger R.D.,
Kniel K.E.,
Calci K.R.,
Hicks D.T.,
Pivarnik L.F.,
Hoover D.G.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.03064.x
Subject(s) - hydrostatic pressure , serotype , microbiology and biotechnology , chemistry , biology , thermodynamics , physics
Aims: The pressure responses of four genotypes of F‐specific RNA bacteriophages, f2, GA, Qβ and SP, were evaluated with respect to pressure magnitude, treatment temperature and suspending medium. Method and Results: The pressure responses were studied with respect to pressure magnitude (350 to 600 MPa), treatment temperature (−10 to 50°C) and suspending media. Phages f2 and GA had much higher pressure resistances than Qβ and SP. Pressure resistances of Qβ and SP were enhanced with increase in salt concentrations in the range of 350 to 600 MPa from −10 to 50°C in PBS. Qβ and SP had greater pressure resistances when suspended in phosphate‐buffered saline (PBS) with added glucose (5%, w/w), UHT whole milk and Dulbecco's Modified Eagle's Medium plus 10% fetal bovine sera than they did in PBS. Two surfactants, sucrose laurate and monolaurin, and one chelating agent, ethylenediamine tetraacetic acid (EDTA), increased the pressure resistance of Qβ and SP, but had modest effect on either f2 or GA. Conclusions: Four representative F‐specific RNA bacteriophages, f2 (serotype I), GA (serotype II), Qβ (serotype III) and SP (serotype IV) showed different resistances to hydrostatic pressure in the range of 350–600 MPa. Significance and Impact of the Study: This study screened for practical surrogates of HAV for validation of commercial high hydrostatic pressure processing.