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A marked enhancement in phytase production by a thermophilic mould Sporotrichum thermophile using statistical designs in a cost‐effective cane molasses medium
Author(s) -
Singh B.,
Satyanarayana T.
Publication year - 2006
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.02921.x
Subject(s) - phytase , food science , thermophile , plackett–burman design , ammonium sulfate , cane , microbiology and biotechnology , chemistry , biochemistry , biology , enzyme , chromatography , fermentation , sugar
Abstract Aims: Statistical optimization of phytase production by a thermophilic mould Sporotrichum thermophile in a cost‐effective cane molasses medium. Methods and Results: Sporotrichum thermophile secreted phytase in cane molasses medium at 45°C and 250 rev min −1 after 5 days. The important factors identified by Plackett–Burman design (magnesium sulfate, Tween 80, ammonium sulfate and incubation period) were further optimized by response surface methodology (RSM). An overall 107% improvement in phytase production was achieved due to optimization. Supplementation of the medium with inorganic phosphate repressed the enzyme synthesis. When inorganic phosphate was reduced from the cane molasses medium by treatment with calcium chloride, the enzyme production increased. The phytase activity was not affected by the enzyme treatment with trypsin and pepsin. Conclusions: A twofold increase in phytase production was achieved due to optimization using statistical designs in a cost‐effective cane molasses medium. Significance and Impact of the Study: Phytase production was doubled due to optimization. The enzyme, being resistant to trypsin and pepsin, thermostable and acid stable, can find application in animal feed industry for improving nutritional status of the feed and combating environmental phosphorus pollution.