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Diversity of Bacillus thuringiensis strains in the maize and bean phylloplane and their respective soils in Colombia
Author(s) -
Jara S.,
Maduell P.,
Orduz S.
Publication year - 2006
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.02901.x
Subject(s) - bacillus thuringiensis , phyllosphere , biology , cry1ac , culex quinquefasciatus , veterinary medicine , population , botany , microbiology and biotechnology , genetically modified crops , larva , bacteria , gene , medicine , aedes aegypti , biochemistry , transgene , genetics , demography , sociology
Aims: To evaluate the distribution of Bacillus thuringiensis strains from maize and bean phylloplane and their respective soils. Methods and Results: B. thuringiensis was isolated from the phylloplane and soil of maize and bean from three municipalities in Antioquia, Colombia. Ninety six samples of phylloplane and 24 of soil were analyzed. A total of 214 isolates were obtained from 96 phylloplane samples while 59 isolates were recovered from 24 soil samples. Sixty five per cent and 12% of the phylloplane and soil isolates, respectively, showed activity against Spodoptera frugiperda . These isolates contained δ‐endotoxin proteins of 57 and 130 kDa. The most toxic isolates against S. frugiperda had the genotype cry1Aa , cry1Ac , cry1B , and cry1D . In contrast, 27% of the phylloplane isolates and 88% of the soil isolates were active against Culex quinquefasciatus and had protein profiles similar to B. thuringiensis serovar. medellin and B. thuringiensis serovar. israelensis . The most active isolates contain cry4 and cry11 genes. Conclusions: The predominant population of B. thuringiensis on the phylloplane harbored the cry1 gene and was active against S. frugiperda , whereas in soil, isolates harboring cry11 gene and active against C. quinquefasciatus were the majority. Significance and Impact of the Study: The predominance of specific B. thuringiensis populations, both on the leaves and in the soil, suggests the presence of selection in B. thuringiensis populations on the studied environment.