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Effects of amino acid on morphological development and nucleus formation of arachidonic acid‐producing filamentous micro‐organism, Mortierella alpina
Author(s) -
Koizumi K.,
Higashiyama K.,
Park E.Y.
Publication year - 2006
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2005.02820.x
Subject(s) - hypha , nucleus , arachidonic acid , biology , amino acid , mycelium , biochemistry , botany , chemistry , microbiology and biotechnology , enzyme
Aims: Effects of amino acid on morphological development and nucleus formation of arachidonic acid‐producing filamentous micro‐organism, Mortierella alpina were investigated using flow‐through chamber. Methods and Results: Mortierella alpina CBS 754·68 was cultivated in flow through chamber using nutrient‐rich, minimal and specific amino acid‐containing minimal media. To investigate the effect of amino acid on morphological parameters either 0·28 g l −1 alanine, 0·53 g l −1 sodium glutamate one hydrate or 0·42 g l −1 valine was added to the minimal medium. In a flow‐through chamber, the growth of hyphal elements and nucleus formation of arachidonic acid‐producing fungus M. alpina were studied on‐line, using image analysis techniques. When the Ala‐ and Val‐containing media were used, the hyphal growth units (HGUs) were 90·2 and 86·7 μ m per tip, respectively, which were 2·4‐fold higher than that in the nutrient‐rich medium, indicating that Ala and Val stimulate the elongation of hyphae. The specific nucleus formation rates were Glu‐>Val‐containing media>minimal and nutrient‐rich media>Ala‐containing medium. The nucleus doubling times in Glu‐ and Val‐containing media were 1·9 and 2 h, respectively, which were not significant different. Conclusions: Ala and Val stimulate the elongation of M. alpina hyphae, and nucleus formation rates were Glu‐>Val‐>Ala‐containing media. Significance and Impact of the Study: Formation of fungal morphology and nucleus were shown using the flow‐through chamber coupled with image analysis, which making possible to discuss the relationship between mycelial morphology and nucleus formation of M. alpina .