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The stability of micro‐organisms preserved in LENTICULE discs, demonstrated by repeat sample distributions of the EQUAL Scheme for Indicator Organisms (water microbiology)
Author(s) -
Boyd P.,
Eaton S.,
Magee J.
Publication year - 2006
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2005.02774.x
Subject(s) - microbiology and biotechnology , biology , sample (material) , chemistry , chromatography
Aims: To demonstrate the stability of microorganisms preserved in LENTICULE discs, employed by the EQUAL Scheme for Indicator Organisms. Methods and Results: Five sample batches containing several indicator organism parameters were each despatched in two separate distributions at intervals of up to 24 months apart. A comparison of participants’ median counts and bar charts of their cumulated results was then made. Conclusions: The results demonstrated that LENTICULE discs provided a remarkably stable preparation format for the maintenance of viable organisms in reproducible numbers. Significance and Impact of the Study: Complex mixtures of organisms can be maintained in stable numbers for extended periods of time at a freezer temperature of −20°C and satisfactorily transported without the requirement for cold chain conditions.