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Anti‐bacterial activity of Lactobacillus plantarum strain SK1 against Listeria monocytogenes is due to lactic acid production
Author(s) -
Wilson A.R.,
Sigee D.,
Epton H.A.S.
Publication year - 2005
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2005.02725.x
Subject(s) - listeria monocytogenes , lactobacillus plantarum , lactic acid , strain (injury) , microbiology and biotechnology , lactobacillus , bacteria , lactobacillaceae , listeria , food science , chemistry , biology , fermentation , anatomy , genetics
Summary Aims: The aim of this research was to investigate the potential of Lactobacillus plantarum strain SK1 for use as a biological control agent against Listeria monocytogenes and determine its mechanism of anti‐listerial activity. Methods and Results: Co‐growth of Lact. plantarum SK1 and L. monocytogenes UMCC98 in MRS broth showed that anti‐listerial activity of Lact. plantarum SK1 occurred during late log/early stationary phase of growth. This coincided with a reduction in broth pH to 4·26. Evidence obtained from the analysis of cell‐free culture filtrates of strain SK1 grown in MRS broth using thin‐layer chromatography and growth of L. monocytogenes in pH‐adjusted culture filtrates suggested that the anti‐listerial activity was due to lactic acid production alone. Trials of Lact. plantarum SK1 on radishes stored at 5°C showed that it had statistically significant ( P < 0·05) anti‐listerial activity. Conclusions: The anti‐listerial activity of Lact. plantarum SK1 was due to lactic acid production alone. A small‐scale trial on radishes stored at 5°C showed it to have significant anti‐listerial activity in planta . Significance and Impact of the Study: This organism has potential as a biological control agent for L. monocytogenes .