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Molecular analysis of microbial population transition associated with the start of denitrification in a wastewater treatment process
Author(s) -
Hoshino T.,
Terahara T.,
Tsuneda S.,
Hirata A.,
Inamori Y.
Publication year - 2005
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2005.02698.x
Subject(s) - denitrifying bacteria , terminal restriction fragment length polymorphism , library , denitrification , restriction fragment length polymorphism , 16s ribosomal rna , biology , bacteria , microbiology and biotechnology , polymerase chain reaction , genetics , chemistry , gene , nitrogen , organic chemistry
Abstract Aims: The objective of this study is to determine the bacteria playing an important role in denitrification by monitoring the molecular dynamics accompanying the start of denitrification. Methods and Results: cDNA reverse‐transcribed from 16S rRNA was amplified with fluorescent labelled primer for terminal restriction fragment length polymorphism (T‐RFLP) analysis and an unlabelled primer for cloning analysis. The terminal restriction fragments (T‐RFs) that increased in association with the start of denitrification were determined. These T‐RFs were identified by in silico analysis of 16S rRNA sequences obtained from cloning. As a result, it was clearly observed that the bacteria belonging to the genera Hydrogenophaga and Acidovorax increased in number after the start of denitrification. Conclusions: It was demonstrated that T‐RFLP analysis targeting 16S rRNA is appropriate for the daily monitoring of a bacterial community to control wastewater treatment processes. Combination of the results of T‐RFLP analysis and 16S rRNA clone library indicated that the bacteria belonging to the genera Hydrogenophaga and Acidovorax play an important role in denitrification. Significance and Impact of the Study: The results of this study provide new insight to the 16S rRNA level of active denitrifying bacteria in wastewater treatment processes.