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Construction of transgenic Bacillus mucilaginosus strain with improved phytase secretion
Author(s) -
Li X.,
Yang S.H.,
Yu X.C.,
Jin Z.X.,
Li W.D.,
Li L.,
Li J.,
Li M.G.
Publication year - 2005
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2005.02683.x
Subject(s) - phytase , strain (injury) , bacillaceae , bacillales , microbiology and biotechnology , secretion , bacillus (shape) , transgene , biology , bacteria , bacillus subtilis , genetics , biochemistry , gene , enzyme , anatomy
Abstract Aims: To construct a transgenic Bacillus mucilaginosus strain to increase the secretion capability of a wild‐type isolate of B. mucilaginosus D 4 B 1 to hydrolyse phytate phosphorus, which can be used as a microbial fertilizer in field application. Methods and Results: We constructed a phytase secreting expression vector pSP43 with a mini‐Tn5 transposon and a Aspergillus fumigatus phytase expression cassette. The vector pSP43 was successfully transferred into the wild‐type B. mucilaginosus using the particle bombardment method, and three transgenic strains with a stable copy of phytase expression cassette integrated into the chromosome of the B. mucilaginosus by Tn5 transposition were selected. The phytase activity of the engineered strains increased 36–46‐fold when compared with the wild‐type strain of D 4 B 1 . Conclusions: The A. fumigatus phytase gene can be expressed under the direction of p43 promoter in B. mucilaginosus. The expression protein is secreted extracellularly and newly constructed strains showed a high phytase activity. Significance and Impact of the Study: A transgenic Bacillus strain by the particle bombardment method was constructed.