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Molecular beacon: a multitask probe
Author(s) -
Goel G.,
Kumar A.,
Puniya A.K.,
Chen W.,
Singh K.
Publication year - 2005
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2005.02663.x
Subject(s) - library science , clinical microbiology , biology , computer science , microbiology and biotechnology
The specificity of hybridization of complementary sequences in DNA is the basic strategy for identifying target genes. For this, stem loop oligonucleotide probes have been developed in order to enhance the specificity and selectivity to the target DNA. Among stem loop oligonucleotides, molecular beacons are the recent probes used for biomolecular recognition reactions. Molecular beacon-based assays are fast, simple, inexpensive, and enable real-time monitoring of nucleic acid reactions both, in vivo and in vitro. This review has been designed to provide a better understanding of the different aspects of molecular beacons, e.g. structure, designing and applications in real-time monitoring of nucleic acid amplification, detection of pathogens, nucleic acid–protein interaction, genetic analysis and array technology.