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Bacterial population in traditional sourdough evaluated by molecular methods
Author(s) -
Randazzo C.L.,
Heilig H.,
Restuccia C.,
Giudici P.,
Caggia C.
Publication year - 2005
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2005.02624.x
Subject(s) - biology , lactobacillus fermentum , lactobacillus , temperature gradient gel electrophoresis , population , 16s ribosomal rna , lactobacillus salivarius , lactobacillaceae , food science , microbiology and biotechnology , bacteria , lactic acid , lactobacillus plantarum , genetics , demography , sociology
Aims: To study the microbial communities in artisanal sourdoughs, manufactured by traditional procedure in different areas of Sicily, and to evaluate the lactic acid bacteria (LAB) population by classical and culture‐independent approaches. Methods and Results: Forty‐five LAB isolates were identified both by phenotypic and molecular methods. The restriction fragment length polymorphism and 16S ribosomal DNA gene sequencing gave evidence of a variety of species with the dominance of Lactobacillus sanfranciscensis and Lactobacillus pentosus , in all sourdoughs tested. Culture‐independent method, such as denaturing gradient gel electrophoresis (DGGE) of the V6–V8 regions of the 16S rDNA, was applied for microbial community fingerprint. The DGGE profiles revealed the dominance of L. sanfranciscensis species. In addition, Lactobacillus ‐specific primers were used to amplify the V1–V3 regions of the 16S rDNA. DGGE profiles flourished the dominance of L. sanfranciscensis and Lactobacillus fermentum in the traditional sourdoughs, and revealed that the closely related species Lactobacillus kimchii and Lactobacillus alimentarius were not discriminated. Conclusions: Lactobacillus ‐specific PCR‐DGGE analysis is a rapid tool for rapid detection of Lactobacillus species in artisanal sourdough. Significance and Impact of the Study: This study reports a characterization of Lactobacillus isolates from artisanal sourdoughs and highlights the value of DGGE approach to detect uncultivable Lactobacillus species.