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Characterization of new O3:K6 strains and phylogenetically related strains of Vibrio parahaemolyticus isolated in Taiwan and other countries
Author(s) -
Wong H.C.,
Chen C.H.,
Chung Y.J.,
Liu S.H.,
Wang T.K.,
Lee C.L.,
Chiou C.S.,
Nishibuchi M.,
Lee B.K.
Publication year - 2005
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2004.02478.x
Subject(s) - vibrio parahaemolyticus , virulence , biology , microbiology and biotechnology , strain (injury) , clone (java method) , gene , hemolysin , vibrionaceae , genetics , bacteria , anatomy
Aims: We analysed the genetic divergence in the pandemic new O3:K6 and phylogenetically related (new O3:K6‐like) strains and compare these two groups in terms of virulence and other biological traits. Methods and Results: A total of 160 new O3:K6, new O3:K6‐like and other strains of Vibrio parahaemolyticus isolated in Taiwan and other countries were collected and their clonal relationships analysed using Sfi I‐pulsed‐field gel electrophoresis. All of the new O3:K6 and new O3:K6‐like strains were grouped in cluster I with five new patterns identified. A O6:K18 strain was identified as a new member of the new O3:K6‐like strains in addition to O4:K68, O1:KUT and O1:K25 strains. All of the lipopolysaccharide preparations of the selected strains exhibited closely spaced quadruplet banding patterns with similar mobility. The two groups of strains exhibited 100% sequence identity in the internal sequences of the toxR and laf genes, and also displayed similar virulence properties as determined with a suckling mouse model. Conclusions: The new O3:K6 and new O3:K6‐like strains were highly similar in virulence and in several other phenotypical and genotypical traits. Significance and Impact of the Study: This work demonstrated the spread and divergence of the pandemic and related clone of V. parahaemolyticus with similar virulence.