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Population structure of Salmonella investigated by amplified fragment length polymorphism
Author(s) -
Torpdahl M.,
Ahrens P.
Publication year - 2004
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2004.02337.x
Subject(s) - amplified fragment length polymorphism , salmonella enterica , biology , bglii , polyphyly , salmonella , subspecies , genetics , monophyly , population , serotype , microbiology and biotechnology , phylogenetics , gene , restriction enzyme , bacteria , zoology , genetic diversity , bamhi , clade , demography , sociology
Abstract Aims: This study was undertaken to investigate the usefulness of amplified fragment length polymorphism (AFLP) in determining the population structure of Salmonella . Methods and Results: A total of 89 strains were subjected to AFLP analysis using the enzymes Bgl II and Bsp DI, a combination that is novel in Salmonella . Both species S. bongori and S. enterica and all subsp. of S. enterica were represented with emphasis on S. enterica subsp. enterica using a local strain collection and strains from the Salmonella Reference Collection B (SARB). The amplified fragments were used in a band‐based cluster analysis. The tree resulting from the subgroup analysis clearly separated all subgroups with high bootstrap values with the species S. bongori being the most distantly related of the subgroups. The tree resulting from the analysis of the SARB collection showed that some serotypes are very clonal whereas others are highly divergent. Conclusions: AFLP clearly clustered strains representing the subgroups of Salmonella together with high bootstrap values and the serotypes of subspecies enterica were divided into polyphyletic or monophyletic types corresponding well with multilocus enzyme electrophoresis (MLEE) and sequence‐based studies of the population structure in Salmonella . Significance and Impact of the Study: AFLP with the enzyme combination Bgl II and Bsp DI allows discrimination of individual strains and provides evidence for the usefulness of AFLP in studies of population structure in Salmonella .