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Identification of waterborne bacteria by the analysis of 16S–23S rRNA intergenic spacer region
Author(s) -
PérezLuz S.,
Adela Yáñez M.,
Catalán V.
Publication year - 2004
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2004.02288.x
Subject(s) - ribosomal intergenic spacer analysis , intergenic region , 23s ribosomal rna , 16s ribosomal rna , biology , ribosomal rna , population , identification (biology) , bacteria , spacer dna , dna sequencer , dna , microbiology and biotechnology , genetics , botany , dna sequencing , genome , rna , internal transcribed spacer , gene , ribosome , demography , sociology
Aim: In this study, we evaluated, the use of universal primers, specific for the 16S‐23S rRNA intergenic region, to detect and identify nine species that are of high interest for the microbiological control of water. Methods and Results: The analysis of the fragments was carried out using a High Resolution acrylamide/bisacrylamide gels in a fluorescent automated DNA sequencer. The results showed specific profiles for each of the nine species but this technique failed to detect simultaneously micro‐organisms in samples containing a mixed population. Conclusion: Nevertheless, the electrophoretic profiles obtained provided a very useful tool for the rapid and specific identification of water isolates. Significance and impact of the study: A possible new methodology for a rapid identification of pathogens in water.