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Protective immunity of SpaA‐antigen producing Lactococcus lactis against Erysipelothrix rhusiopathiae infection
Author(s) -
Cheun H.I.,
Kawamoto K.,
Hiramatsu M.,
Tamaoki H.,
Shirahata T.,
Igimi S.,
Makino S.I.
Publication year - 2004
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2004.02283.x
Subject(s) - erysipelothrix rhusiopathiae , lactococcus lactis , microbiology and biotechnology , vaccination , antigenicity , biology , antigen , erysipelas , streptococcaceae , lactococcus , virology , bacteria , medicine , immunology , lactic acid , antibiotics , genetics , pathology
Aims: To develop an economical, safe and simple vaccination system against swine erysipelas using SpaA‐antigen producing Lactococcus lactis . Methods and Results: The spaA gene of Erysipelothrix rhusiopathiae was inserted into a shuttle plasmid pSECE1 to construct pSECE1·3. The SpaA produced in L. lactis maintained a stable antigenicity without degrading in growth. After mice were inoculated intranasally and orally with pSECE1·3‐carrying L. lactis cells, IgG and IgA specific to SpaA were detected, and all the mice survived a challenge with 100 LD 50 of E. rhusiopathiae Tama‐96 in the inner thigh. Conclusions: SpaA‐producing L. lactis appears useful as an effective subunit vaccine against swine erysipelas. Significance and Impact of the Study: In this vaccination system, purification of the antigen and injection are unnecessary, leading to a reduced production cost, reduced labour and less stress to the animals. This vaccination system of the lactic acid bacteria should be a safe and suitable vehicle for a polyvalent vaccine.