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The expression of an R3 lipopolysaccharide‐core by pathotypes of Escherichia coli
Author(s) -
Chart H.,
Perry N.T.,
Jenkins C.
Publication year - 2004
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2004.02233.x
Subject(s) - escherichia coli , vtec , microbiology and biotechnology , verocytotoxin , biology , enteroaggregative escherichia coli , serotype , amplicon , lipopolysaccharide , virulence , enterobacteriaceae , virology , polymerase chain reaction , gene , genetics , endocrinology
Aims: To investigate the incidence of an R3 lipopolysaccharide (LPS)‐core amplicon in a range of pathotypes of Escherichia coli , including Verocytotoxin‐producing E. coli (VTEC), enteroaggregative E. coli (EAggEC) and enteropathogenic E. coli (EPEC). Methods and Results: A total of 100 strains of E. coli belonging to a range of pathotypes, including 41 strains of VTEC, were screened for the genes encoding the R3 LPS‐core using PCR. Fifty‐four per cent produced an amplicon with the R3 primer set. Of the 41 VTEC, 66% had an R3 LPS‐core with a PCR product being observed with all strains belonging to serotypes O26:H11, O111ac:H‐ and O145:H25. However, 46% of enteroaggregative E. coli and 50% of enteropathogenic E. coli were also shown to have an R3 LPS‐core structure. Conclusions: Strains with an R3 LPS‐core are widely distributed within the species E. coli. Significance and Impact of the Study: Strains of E. coli with an R3 LPS‐core structure appear not to be associated with a specific pathotype.