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Evaluation of a RAPD‐based typing scheme in a molecular epidemiology study of Vibrio cholerae O1, Brazil
Author(s) -
Leal N.C.,
Sobreira M.,
LealBalbino T.C.,
Almeida A.M.P.,
Silva M.J.B.,
Mello D.M.,
Seki L.M.,
Hofer E.
Publication year - 2004
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2004.02090.x
Subject(s) - rapd , vibrio cholerae , biology , typing , serotype , microbiology and biotechnology , molecular epidemiology , genetics , virulence , el tor , outbreak , gene , genetic diversity , virology , genotype , bacteria , medicine , population , environmental health
Aims: To evaluate the utility of random amplification of polymorphic DNA (RAPD) technique for routine practice in public health laboratories for epidemiological studies of Vibrio cholerae O1 isolates. Materials and Results: Seventy‐nine strains were examined by PCR for the toxin genes ( ctx A, zot and ace ), virulence‐associated genes ( tcp A and tox T) and RAPD sequences. Except for one strain (no. 1123) from the Amazonas State, all the strains analysed carried the genes ctx A, zot , ace , tcp A and tox T. RAPD fingerprinting revealed variability but no correlation with serotype, biotype or geographical origin of the isolates was found. Conclusion: A standardized RAPD method does not enable the establishment of a pattern data bank for the identification of V. cholerae O1 strains. Significance and Impact of the Study: The simplicity and discriminative capacity of this technique make it useful for detecting genetic diversity among micro‐organisms from a defined group or for outbreak investigation.