Evaluation of a RAPD‐based typing scheme in a molecular epidemiology study of  Vibrio cholerae  O1, Brazil | Zendy

Leal N.C. | Zendy; Sobreira M. | Zendy; LealBalbino T.C. | Zendy; Almeida A.M.P. | Zendy; Silva M.J.B. | Zendy; Mello D.M. | Zendy; Seki L.M. | Zendy; Hofer E. | Zendy

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Evaluation of a RAPD‐based typing scheme in a molecular epidemiology study of Vibrio cholerae O1, Brazil

Author(s) -

Leal N.C.,

Sobreira M.,

LealBalbino T.C.,

Almeida A.M.P.,

Silva M.J.B.,

Mello D.M.,

Seki L.M.,

Hofer E.

Publication year - 2004

Publication title -

journal of applied microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.889

H-Index - 156

eISSN - 1365-2672

pISSN - 1364-5072

DOI - 10.1111/j.1365-2672.2004.02090.x

Subject(s) - rapd , vibrio cholerae , biology , typing , serotype , microbiology and biotechnology , molecular epidemiology , genetics , virulence , el tor , outbreak , gene , genetic diversity , virology , genotype , bacteria , medicine , population , environmental health

Aims: To evaluate the utility of random amplification of polymorphic DNA (RAPD) technique for routine practice in public health laboratories for epidemiological studies of Vibrio cholerae O1 isolates. Materials and Results: Seventy‐nine strains were examined by PCR for the toxin genes ( ctx A, zot and ace ), virulence‐associated genes ( tcp A and tox T) and RAPD sequences. Except for one strain (no. 1123) from the Amazonas State, all the strains analysed carried the genes ctx A, zot , ace , tcp A and tox T. RAPD fingerprinting revealed variability but no correlation with serotype, biotype or geographical origin of the isolates was found. Conclusion: A standardized RAPD method does not enable the establishment of a pattern data bank for the identification of V. cholerae O1 strains. Significance and Impact of the Study: The simplicity and discriminative capacity of this technique make it useful for detecting genetic diversity among micro‐organisms from a defined group or for outbreak investigation.

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