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Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders
Author(s) -
Brenner V.,
Rucká L.,
Totevová S.,
Tømeraas K.,
Demnerová K.
Publication year - 2004
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2004.02075.x
Subject(s) - complementation , strain (injury) , pseudomonas fluorescens , degradation (telecommunications) , biphenyl , pseudomonas , chemistry , biodegradation , burkholderia , biochemistry , biology , bacteria , microbiology and biotechnology , gene , organic chemistry , mutant , genetics , anatomy , telecommunications , computer science
Aims: A possibility for the complementation of both ortho ‐ and meta ‐cleavage pathway for chlorocatechols in one strain and its impact on degradation of chlorobenzoates accumulated during degradation of polychlorinated biphenyls was investigated. Methods and Results: Genes responsible for ortho ‐cleavage of chlorocatechols were subcloned into two biphenyl degraders and the activities of chlorocatechol dioxygenases responsible for ortho ‐ and meta ‐cleavage in these hybrid strains were monitored spectrophotometrically and also electrochemically by ion‐selective electrode. Conclusions: While strain Pseudomonas fluorescens S12/C apparently gained metabolic advantage from this gene manipulation, strain Burkholderia cepacia P166/C did not express better degradation features in comparison with the parental strain. Significance and Impact of the Study: This approach has the potential to enhance chlorocatechol metabolism in selected biphenyl degraders.