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Note: Characterization of Vibrio cholerae O139 Bengal isolated from water in Malaysia
Author(s) -
Son R.,
Rusul G.,
Samuel L.,
Senthil S.,
Rasip A.,
Nasreldin E.H.,
Nishibuchi M.
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.1998.tb05273.x
Subject(s) - vibrio cholerae , microbiology and biotechnology , cholera toxin , biology , rapd , plasmid , cholera , toxin , vibrionaceae , gene , bengal , antibiotic resistance , antibiotics , genetics , genetic diversity , bacteria , medicine , geography , archaeology , bay , population , environmental health
R. SON, G. RUSUL, L. SAMUEL, YUHERMAN, S. SENTHIL, A. RASIP, E.H. NASRELDIN AND M. NISHIBUCHI. 1998. Four Vibrio cholerae O139 Bengal strains isolated from surface water were characterized by antibiotic resistance, plasmid profile, presence of cholera toxin gene and random amplification of polymorphic DNA (RAPD) analysis. All four strains exhibit multiple resistance towards the antibiotics tested with a multiple antibiotic resistance index of 0·5–0·66, and harboured a 2·0 MDa non‐conjugative plasmid. The Vibrio cholerae O139 Bengal were positive for the cholera toxin gene. Antibiotyping and random amplification of polymorphic DNA analysis with four primers proved to be useful in discriminating the isolates. RAPD proved to be more sensitive. These results reveal that there is significant genetic diversity among the Vibrio cholerae O139 Bengal strains studied.