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Phospholipid molecular species distribution of Porphyromonas asaccharolytica ATCC 25260 T : effects of temperature, culture age and pH
Author(s) -
Tavana A.M.,
Drucker D.B.,
Boote V.
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.1998.tb05268.x
Subject(s) - phospholipid , incubation , chemistry , anaerobic exercise , mass spectrometry , chromatography , biology , biochemistry , physiology , membrane
A.M. TAVANA, D.B. DRUCKER AND V. BOOTE. 1998. The aim of this study was to determine the extent to which phospholipid molecular species profiles are affected by different environmental factors in Porphyromonas asaccharolytica ATCC 25260 T . Phospholipids were analysed by Fast Atom Bombardment Mass Spectrometry (FAB‐MS) in negative‐ion mode. Under standard growth conditions (37°C, pH7·0, 48 h), the most intense high mass anions were m/z 653 and 662. The latter is consistent with the expected presence of PE (30:0). The only changes in profiles were quantitative. These were compared using the Pearson Coefficient of Linear Correlation. The r ‐values for initial pH comparisons ranged from 0·82 (pH 7·0 vs pH 6·0) to 1·00 (pH 5·0 vs pH 8·0), for incubation period, from 0·86 (48 vs 72 h) to 0·97 (96 vs 168 h), and for temperature, from 0·57 (40 vs 37°C) to 0·96 (37 vs 36°C). Differences were also seen when plates were incubated in anaerobe jars as opposed to an anaerobic work station ( r = 0·75). It is concluded that it is essential to standardize growth parameters, and to use an anaerobe jar or an anaerobe work station, but not both.