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Degradation of fluoranthene by Pasteurella sp. IFA and Mycobacterium sp. PYR‐1: isolation and identification of metabolites
Author(s) -
Šepič E.,
Bricelj M.,
Leskovšek H.
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.1998.00587.x
Subject(s) - fluoranthene , biodegradation , chemistry , chromatography , derivatization , phenylacetic acid , benzoic acid , bacteria , microbiology and biotechnology , biochemistry , organic chemistry , biology , high performance liquid chromatography , phenanthrene , genetics
The findings from a biodegradability study of fluoranthene using two pure bacterial strains, Pasteurella sp. IFA (B‐2) and Mycobacterium sp. PYR‐1 (AM) are reported. Of total fluoranthene, 24% (B‐2) and 46% (AM) was biodegraded in an aqueous medium during 14 d of incubation at room temperature. During this period the bacteria were capable of mineralizing approximately two‐thirds (B‐2) and four‐fifths (AM) of biodegraded fluoranthene to CO 2 , while one‐third (B‐2) and one‐fifth (AM) of the original fluoranthene remained as stable metabolic products. These metabolites were isolated using liquid–liquid extraction and identified using gas chromatography – mass spectrometry (GC–MS) and derivatization techniques. Two metabolites (9‐fluorenone‐1‐carboxylic acid and 9‐fluorenone) were identified by GC–MS directly, while the metabolites 9‐fluorenone‐1‐carboxylic acid, 9‐hydroxyfluorene, 9‐hydroxy‐1‐fluorene‐carboxylic acid, 2‐carboxybenzaldehyde, benzoic acid and phenylacetic acid were determined in their derivatized forms. From the identified metabolites, a fluoranthene biodegradation pathway was proposed for Pasteurella sp. IFA.