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Genetic relationships among Pseudomonas stutzeri strains based on molecular typing methods
Author(s) -
Bennasar A.,
Guasp C.,
Tesar M.,
Lalucat J.
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.1998.00572.x
Subject(s) - pseudomonas stutzeri , biology , library science , humanities , computer science , philosophy , genetics , bacteria
Detailed characterization of the genetic variability among strains belonging to Pseudomonas stutzeri was achieved using different rapid molecular typing methods based on polymerase chain reaction (PCR), Southern blot and Western blot. Consensus motifs complementary to fragments of repetitive elements dispersed throughout the genomes of bacteria were used as primers & allowed differentiation at subspecies levels. Further & simple differentiation was also achieved based on the direct amplification of spacer regions between 16S & 23S rRNA, combined with single‐strand conformation polymorphism (SSCP) analysis of the generated fragments. These methods are fast, sensitive, reliable for determining relationships & have demonstrated a great genetic diversity among the strains of Ps. stutzeri studied in agreement with the heterogeneous phenotypic traits of the species.