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Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production
Author(s) -
Gromada A.,
Fiedurek J.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.1997.tb03597.x
Subject(s) - ethidium bromide , aspergillus niger , mutant , extracellular , glucose oxidase , strain (injury) , enzyme , biochemistry , biology , mutation , mutagenesis , chemistry , microbiology and biotechnology , gene , dna , anatomy
After mutagenization and selection, mutant Aspergillus niger strains resistant to certain agents were obtained. Seven of the mutants showed increased extracellular glucose oxidase (GOD), the level for individual cases ranged widely from 8.8 to over 138.5% in comparison with the parental strain. Studies of the relationship between method of selection and frequency of mutation showed that the highest frequency of positive mutations (15.8% and 17.3%) was obtained from mutants resistant to ethidium bromide (1 mmol 1 ‐1 ) and sodium gluconate (45%), respectively. The time course of growth and enzyme production by the most active mutant AM‐11 showed intra‐ and extracellular GOD activities to have increased about 2.2‐ and 2.4‐fold, respectively, compared with the parental strain.