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Microtitre plate hybridization system for detection of thermophilic Campylobacter rRNA
Author(s) -
Lamoureux M.,
Fliss I.,
Blais B.W.,
Messier S.,
Holley R.A.,
Simard R.E.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.1997.tb03582.x
Subject(s) - thermophile , campylobacter , biology , microbiology and biotechnology , chemistry , bacteria , genetics
A microtitre plate nucleic acid probe hybridization system was developed for the detection of ribosomal RNA from thermophilic Campylobacter (Camp. jejuni, Camp. coli, Camp. lari and Camp. upsaliensis). A specific DNA probe obtained by amplification of 23S rRNA sequences using the polymerase chain reaction technique was immobilized on a microtitre plate, and used for hybridization with target 23S rRNA from cell lysates. The RNA‐DNA hybrids thus formed in the wells were detected by an immunoenzymatic assay using a monoclonal antiRNA‐DNA hybrid antibody. The sensitivity of this system was 2.7 x 10 4 cells ml ‐1 . This simple, sensitive and inexpensive hybridization and immunoenzymatic assay system should facilitate the detection of Campylobacter in food and clinical samples.