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Invasion of bovine epithelial cells by verocytotoxin‐producing Escherichia coli O157: H7
Author(s) -
Matthews K.R.,
Murdough P.A.,
Bramley A.J.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.1997.tb03573.x
Subject(s) - verocytotoxin , vtec , escherichia coli , biology , hela , shiga like toxin , microbiology and biotechnology , cell culture , plasmid , vero cell , toxin , gentamicin protection assay , enterobacteriaceae , gene , genetics , western blot
The ability of verocytotoxin‐producing Escherichia coli (VTEC) O157: H7 to enter selected human (RPMI‐4788 and HeLa) and bovine (MAC‐T, mammary secretory; MDBK, kidney) epithelial cell lines was evaluated. All VTEC evaluated efficiently entered RPMI‐4788 and MAC‐T cell lines. VTEC entered MDBK cells at approximately 4% of MAC‐T cells. VTEC were not able to invade HeLa cells. Presence of plasmid had no influence on efficiency of entry, nor did production of shiga‐like toxin (SLT I or SLT II). Internalization required microfilaments, but not microtubules. Two types of adherence, localized and diffuse, were exhibited depending on isolate and cell line evaluated. Ability of VTEC to invade bovine mammary epithelial cells may be important in pathogenesis in the bovine, may indicate a route by which raw milk may potentially become contaminated, and may provide a reservoir of bacteria for the contamination of workers, equipment and carcass at time of slaughter.