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Effect of pH, temperature and culture medium composition on the production of an extracellular cysteine proteinase by Micrococcus sp. INIA 528
Author(s) -
Mohedano A.F.,
Fernández J.,
Gaya P.,
Medina M.,
Nuñez M.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.1997.tb03300.x
Subject(s) - yeast extract , food science , enzyme , fermentation , composition (language) , enzyme assay , biology , micrococcus , extracellular , growth medium , chemistry , biochemistry , bacteria , linguistics , philosophy , genetics
Growth and proteinase production by Micrococcus sp. INIA 528 in a batch‐operated laboratory fermentor were investigated, with trypticase soy broth as the basal medium for studies on optimum temperature, pH and medium composition. Maximum growth was recorded at 34°C and pH 715, whereas optimum temperature and pH for proteinase production were 31°C and pH 6.25. Maximum rate of enzyme production occurred during the late log and early stationary phases of growth. Addition of 5.0 g 1 ‐1 yeast extract, 1.0 g 1 ‐1 glucose, 1.0 g 1 ‐1 MgSO 4 or 1.0 g 1 ‐1 K 2 HPO 4 to basal medium resulted in a lower enzyme yield, but supplementation of basal medium with 2.5 g 1 ‐1 (NH 4 ) 2 SO 4 increased enzyme production by 45%. A high initial biomass added to fresh broth supplemented with 2.5 g 1 ‐1 (NH 4 ) 2 SO 4 only increased enzyme activity by 19%, compared to the maximum enzyme activity achieved with the standard inoculum.