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Note : Inhibition of the growth of Yersinia enterocolitica O:3 by the microflora of porcine caecum and ileum in an in vitro model
Author(s) -
Asplund K.,
Hakkinen M.,
Björkroth J.,
Nuotio L.,
Nurmi E.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb04504.x
Subject(s) - yersinia enterocolitica , caecum , ileum , microbiology and biotechnology , virulence , population , yersinia , biology , bacteria , in vitro , enterobacteriaceae , plasmid , escherichia coli , biochemistry , medicine , environmental health , gene , genetics , dna
The growth of Yersinia enterocolitica O:3 was tested in an in vitro model of the porcine intestine at the physiological temperature of 39°C of growing pigs. The model supported a stable population of Y. enterocolitica at a level 10 8 –10 9 cells ml ‐1 . Plasmid profile analysis and the Ca 2+ ‐dependent proportion of the population suggested that the great majority of the Y. enterocolitica population retained the 70 kb virulence plasmid, pYV, throughout the experimental period of 5 d. The growth of Y. enterocolitica was substantially inhibited by the ileal and the caecal flora compared to the growth of the bacterium alone. Yersinia enterocolitica was not isolated after 3 d of cultivation.