Premium
A comparison between a PCR method and a conventional culture method for detecting pathogenic Yersinia enterocolitica in food
Author(s) -
Lambertz S. Thisted,
BallagiPordány A.,
Nilsson A.,
Norberg P.,
DanielssonTham M.L.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb04332.x
Subject(s) - yersinia enterocolitica , virulence , polymerase chain reaction , biology , locus (genetics) , microbiology and biotechnology , yersinia , virulence factor , plasmid , pathogenic bacteria , gene , genetics , bacteria
S. THISTED LAMBERTZ, A. BALLAGI‐PORDÁNY, A. NILSSON, P. NORBERG AND M.‐L. DANIELSSON‐THAM. 1996. The aim of this study was to develop a polymerase chain reaction (PCR) method for the detection of pathogenic Yersinia enterocolitica and to compare it with an official culture method (NMKL‐117). Primers were selected for nested PCR directed at the attachment invasion locus, ail , on the bacterial chromosome, as well as at a sequence on the pathogenic marker plasmid, termed virulence factor, virF. The final results obtained by the two methods were similar. However, while the conventional method yielded contradictory data for some steps the PCR method provided unambiguous results. Considerable advantages, i.e. higher sensitivity and specificity of the PCR method, compared with the conventional method for detecting pathogenic Y. enterocolitica , were demonstrated in this study.