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Heat‐stable serotyping antigens expressed by strains of Campylobacter jejuni are probably capsular and not long‐chain lipopolysaccharide
Author(s) -
Chart H.,
Frost J.A.,
Oza A.,
Thwaites R.,
Gillanders S.,
Rowe B.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb03558.x
Subject(s) - campylobacter jejuni , serotype , lipopolysaccharide , microbiology and biotechnology , antigen , campylobacter , biology , bacteria , virology , immunology , genetics
The role of lipopolysaccharide (LPS) in the serotyping of Campylobacter jejuni based on heat‐stable antigens was examined using SDS‐PAGE and a silver stain for carbohydrate. None of the 32 type strains of Camp. jejuni expressed long‐chain LPS. Rabbit antibodies, prepared to 10 selected strains of Camp. jejuni , reacted with surface‐exposed carbohydrate antigens, which were not LPS. This study suggests that the heat‐stable antigens of Camp. jejuni , which form the basis for the established Penner serotyping scheme, are probably capsular and not LPS.