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Plasmid characterization and pulsed‐field electrophoretic analysis demonstrate that ampicillin‐resistant strains of Salmonella enteritidis phage type 6a are derived from Salm. enteritidis phage type 4
Author(s) -
Ridley A.M.,
Punia P.,
Ward L.R.,
Rowe B.,
Threlfall E.J.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb03555.x
Subject(s) - salmonella enteritidis , plasmid , ampicillin , microbiology and biotechnology , pulsed field gel electrophoresis , biology , amp resistance , salmonella , bacteria , genetics , dna , gene , antibiotics , genotype
Plasmid incompatibility studies have demonstrated that strains of Salmonella enteritidis phage type (PT) 6a resistant to ampicillin possess a 36 megadalton incompatibility group (Inc) X plasmid coding for resistance to ampicillin which is capable of converting strains of Salm. enteritidis belonging to PTs 1 and 4 to PT 6a, and PT 8 to PT 13. However, pulsed‐field gel electrophoresis (PFGE) has demonstrated that all clinical isolates of PT 6a have a characteristic Xba I pulsed‐field profile which is distinct from that of PT 1 and which can only be differentiated from that of PT 4 by the presence of plasmid‐associated fragments of less than 45 kb. It is concluded that ampicillin‐resistant strains of Salm. enteritidis PT 6a are derived from strains of Salm. enteritidis PT 4 by acquisition of an Inc X ampicillin resistance plasmid.