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The use of an automated assay to assess phage survival after a biocidal treatment
Author(s) -
Maillard J.Y.,
Beggs T.S.,
Day M.J.,
Hudson R.A.,
Russell A.D.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb03264.x
Subject(s) - coliphage , escherichia coli , lysis , microbiology and biotechnology , chemistry , bacteriophage , biology , chromatography , biochemistry , gene
The coliphage K 1‐5 has been used in an automated assay to monitor the viricidal activity of various disinfectants. This indirect assay based on the spectrophotometric reading of the lysis of the host cell ( Escherichia coli D837) produced encouraging results and was faster than the overlay counting method (previously studied) which relies on plaque formation. However, differences in sensitivity towards some disinfectants were observed between the two methods.