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Differentiation between Mycobacterium tuberculosis and Mycobacterium bovis by a multiplex‐polymerase chain reaction
Author(s) -
Herrera E.A.,
Pérez O.,
Segovia M.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb03263.x
Subject(s) - mycobacterium tuberculosis , mycobacterium bovis , polymerase chain reaction , mycobacterium tuberculosis complex , multiplex polymerase chain reaction , biology , tuberculosis , mycobacterium , insertion sequence , microbiology and biotechnology , multiplex , virology , gene , bacteria , genetics , medicine , genome , transposable element , pathology
A multiplex‐polymerase chain reaction (PCR) assay, based on one‐step amplification and detection of three different mycobacterial genomic fragments, was designed for differentiation between Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from the gro EL gene, present in the genus Mycobacterium sp., from the IS 6110 insertion sequence, present in Myco. tuberculosis complex and from the mtp 40 gene, identified as a specificspecies Myco. tuberculosis genomic fragment. This amplification method allowed the detection of two fragments of 576 and 317 base pairs in Myco. bovis and three fragments of 576, 396 and 317 base pairs in Myco. tuberculosis strains, including atypical strains of Myco. tuberculosis where the copy number of the IS 6110 element is low. The multiplex‐PCR assay described may be a very useful tool for the rapid and specific differentiation of these related mycobacteria and easy to use in medical and veterinary microbiology laboratories.