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Interaction between fish spoilage bacteria Pseudomonas sp. and Shewanella putrefaciens in fish extracts and on fish tissue
Author(s) -
Gram L.,
Melchiorsen J.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb03262.x
Subject(s) - shewanella putrefaciens , pseudomonas , microbiology and biotechnology , siderophore , shewanella , biology , bacteria , food spoilage , pseudomonadales , pseudomonas aeruginosa , pseudomonadaceae , agar , food science , chemistry , genetics
The interaction between fish spoilage bacteria, Pseudomonas sp. and Shewanella putrefaciens , was investigated using fish extract and fish tissue as model systems. Isolates of Pseudomonas that produced iron chelators, siderophores, inhibited growth of S. putrefaciens in a fish‐extract‐agar diffusion assay but no, or only weak, antagonistic activity was seen when the medium was supplemented with iron. Sterile‐filtered supernatant fluid from a siderophore‐producing Pseudomonas grown in fish extract was inhibitory to S. putrefaciens if the number of Pseudomonas was above 10 8 cfu ml −1 . In contrast, supernatant fluids from siderophore‐negative Pseudomonas isolates did not inhibit growth of S. putrefaciens. The inhibitory effect was, except for one strain of Pseudomonas , not seen in supernatant fluids from iron‐enriched cultures of Pseudomonas sp. Finally, siderophore‐producing Pseudomonas sp. lowered the maximum cell level of S. putrefaciens 1–2 log units from 10 9 to 10 10 cfu g −1 when the strains were grown on fish muscle blocks at 0°C but the growth rate of S. putrefaciens was not affected.