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Effect of two levels of transgalactosylated oligosaccharide intake in rats associated with human faecal microflora on bacterial glycolytic activity, end‐products of fermentation and bacterial steroid transformation
Author(s) -
Kikuchi H.,
Andrieux C.,
Riottot M.,
Bensaada M.,
Popot F.,
Beaumatin P.,
Szylit O.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb03240.x
Subject(s) - fermentation , lactic acid , biology , steroid , hindgut , biochemistry , bacteria , oligosaccharide , food science , chemistry , genetics , botany , larva , hormone , midgut
The effects of two levels of transgalactosylated oligosaccharide (TOS) intake on bacterial glycolytic activity, end products of fermentation and bacterial steroid transformation were studied in rats associated with a human faecal flora. Rats were fed a human‐type diet containing 0, 5 or 10% TOS. Caecal pH decrease correlated with the amount of TOS in the diet. Intake of the TOS diet induced a decrease in blood cholesterol and a strong increase in β‐galactosidase activity in the hindgut. TOS fermentation led to production of hydrogen and short chain fatty acids, whereas ammonia and branched‐chain fatty acids were decreased. A diet containing 10% TOS increased caecal lactic acid concentrations and reduced β‐glucuronidase activities and steroid transformation.