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Uptake and utilization of n ‐octacosane and n ‐nonacosane by Arthrobacter nicotianae KCC B35
Author(s) -
Radwan S.S.,
Sorkhoh N.A.,
Felzmann H.,
ElDesouky A.F.
Publication year - 1996
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1996.tb03231.x
Subject(s) - pentadecane , hexadecane , hydrocarbon , chemistry , arthrobacter , carbon chain , carbon fibers , alkenone , food science , fatty acid , botany , organic chemistry , environmental chemistry , biology , paleontology , materials science , composite number , composite material , enzyme , holocene
Arthrobacter nicotianae KCC B35 isolated from blue‐green mats densely covering oil sediments along the Arabian Gulf coast grew well on C 10 to C 40 n ‐alkanes as sole sources of carbon and energy. Growth on C 20 to C 40 alkanes was even better than on C 10 to C 18 alkanes. Biomass samples incubated for 6 h with n ‐octacosane (C 28 ) or n ‐nonacosane (C 29 ) accumulated these compounds as the predominant constituent alkanes of the cell hydrocarbon fractions. The even chain hexadecane C 16 and the odd chain pentadecane C 15 were the second dominant constituent alkanes in C 28 and C 29 incubated cells, respectively. n ‐Hexadecane‐incubated cells accumulated in their lipids higher proportions of C 16 ‐fatty acids than control cells not incubated with hydrocarbons. On the other hand, C 28 and C 29 ‐incubated cells did not contain any fatty acids with the equivalent chain lengths, but the fatty acid patterns of the cell lipids suggest that there should have been mid‐chain oxidation of these very long chain alkanes. This activity qualifies A. nicotianae KCC B35 to be used in cocktails for bioremediating environments polluted with heavy oil sediments.