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Evaluation of the MicroScan enzyme‐based system for the identification of foodborne yeasts
Author(s) -
Deak T.,
Beuchat L. R.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb03159.x
Subject(s) - identification (biology) , food science , biology , microbiology and biotechnology , botany
Eighty‐nine strains representing 36 species of foodborne yeasts isolated from fruit juice concentrates were identified using the Baxter MicroScan enzyme‐based kit, conventional tests according to a simplified identification method (SIM), and the API 20C kit. Of the 15 test species included in the MicroScan database, only 40% were correctly identified; 13% gave scores of unacceptably low probabilities, 20% were misidentified, and 27% could not be identified. Of the 21 test species not in the MicroScan database, 38% were misidentified and 62% produced biocodes with between‐species differences not larger than differences between strains within species. The reliability of the MicroScan enzyme‐based system is questioned, in that different results were sometimes obtained upon retesting the same strains. The MicroScan enzyme‐based system is rapid, providing results within 4 h. However, because of its restricted and specific database and unreliability, the system appears to be unsuited for the identification of foodborne yeasts.