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Plasmid formation and its relation to the formation of spontaneously developing pocks in Streptomyces azureus ATCC 14921
Author(s) -
Doi K.,
Saeki M.C.,
Ono Y.,
Ogata S.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb03132.x
Subject(s) - plasmid , mycelium , biology , streptomyces , sequence (biology) , strain (injury) , streptomycetaceae , genetics , microbiology and biotechnology , actinomycetales , botany , dna , bacteria , anatomy
Streptomyces azureus ATCC 14921 harboured a plasmid pSA1 together with its chromosomal integrated sequence (pSA1 int ). The att P site on the plasmid was located at ca 170 bp Bam HI‐ Sph I fragment by site‐specific integration. The free form was generated from the integrated sequence during the development of its host mycelia in the solid culture, but not in the liquid culture. The free form seemed to elicit the formation of spontaneously developing pocks on its host mycelia in the solid culture.