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Screening of xylanolytic bacteria using a colour plate method
Author(s) -
Breccia J.D.,
Castro G.R.,
Baigorí M.D.,
Siñeriz F.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb03086.x
Subject(s) - hemicellulose , bacteria , petri dish , bagasse , isolation (microbiology) , food science , substrate (aquarium) , microbiology and biotechnology , chemistry , hydrolysis , biology , biochemistry , ecology , genetics
A simple procedure is described for the screening and isolation of xylanolytic bacteria, able to degrade hemicellulose. Bagasse hemicellulose was purified, cross‐linked with green dye for use as indicator, and used in a Petri dish assay for the determination of xylanolytic activity. Hydrolysis of the substrate causes the formation of transparent halos around bacterial colonies. The screening procedure was used to select thermoresistant bacteria. The four best isolates were characterized and classified as Bacillus species.