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Non‐culturable Legionella pneumophila associated with Acanthamoeba castellanii: detection of the bacterium using DNA amplification and hybridization
Author(s) -
Hay J.,
Seal D.V.,
Billcliffe B.,
Freer J.H.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb01674.x
Subject(s) - legionella pneumophila , microbiology and biotechnology , acanthamoeba , biology , legionella , bacteria , agar , hybridization probe , agar plate , dna–dna hybridization , dna , genetics
J. HAY. D.V. SEAL, B.BILLCLIFFE AND J.H. FREER. 1995. The intracellular localization of Legionella pneumophila serogroup 1 within Acanthamoeba castellanii rendered the bacteria non‐culturable on supplemented BCYE agar. DNA amplification, using two 19‐mer primers, and hybridization using a 25‐mer oligonucleotide probe, permitted detection of Leg. pneumophila in approximately 81% (29/36) of samples where the bacteria could not be detected using culture. A combination of co‐cultivation of samples with Leg. pneumophila ‐naive A. polyphaga or Hartmannella vermiformis , incubation in a defined liquid medium or use of catalase indicated that approximately 31% (9/29) of the samples contained Leg. pneumophila which were viable although not culturable.