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Comparative evaluation of plasmid profiling and ribotyping in the analysis of Lactobacillus plantarum strain heterogeneity in silage
Author(s) -
Duffner Fiona,
O'Connell M.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb01668.x
Subject(s) - ribotyping , lactobacillus plantarum , biology , silage , microbiology and biotechnology , typing , plasmid , restriction enzyme , population , strain (injury) , bacteria , food science , dna , genetics , polymerase chain reaction , lactic acid , gene , demography , anatomy , sociology
F. DUFFNER AND M. O'CONNELL. 1995. Seventy‐two Lactobacillus plantarum isolates were recovered from six uninoculated grass silages for the purposes of firstly evaluating the usefulness of (1) restriction endonuclease digestion of total genomic DNA, (2) plasmid profiling and (3) ribotyping in Lact. plantarum strain differentiation and secondly, examining the strain heterogeneity in well preserved silage. The three methods for differentiation were applied to 72 of the isolates and allowed at least 32 different strains to be identified. Twenty‐five different plasmid profiles were detected (26 if the absence of plasmids is included as a profile). Ribotyping with Eco RI identified only 11 patterns among the silage isolates. A variety of restriction enzymes was screened to increase the sensitivity of ribotyping to detect strain differences and Bam HI was used successfully for this purpose, differentiating all of the strains tested. Two dominant strains (I and II) were identified in one particular silage, comprising 47% and 17% respectively of the isolates, while strains III and V comprised 37% and 25% of the Lact. plantarum population isolated from another of the silages.