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Continuous production of bacteriocins, brevicin, nisin and pediocin, using calcium alginate‐immobilized bacteria
Author(s) -
Wan J.,
Hickey M. W.,
Coventry M. J.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb00953.x
Subject(s) - bacteriocin , nisin , pediococcus acidilactici , lactococcus lactis , calcium alginate , chemistry , pediococcus , bacteria , lactobacillus , chromatography , food science , lactococcus , calcium , microbiology and biotechnology , lactic acid , fermentation , biology , antimicrobial , lactobacillus plantarum , organic chemistry , genetics
Bacteriocins including brevicin 286, nisin and pediocin PO2 have been produced successfully with immobilized cells of Lactobacillus brevis VB286, Lactococcus lactis subsp. lactis and Pediococcus acidilactici PO2 respectively encapsulated in calcium alginate beads. The beads encapsulating the bacteriocin‐producing cells were loaded into a column, and continuously supplied with fresh medium at a flow rate of 1 bed volume per 20 min. The concentrations of the three bacteriocins produced in the eluents were at least as high as those obtained from conventional free‐cell batch fermentations.