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Genetic and functional analysis of novobiocin non‐producing mutants of Streptomyces niveus
Author(s) -
Hoggarth J. H.,
Cushing K. E.,
Ritchie D. A.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb00946.x
Subject(s) - novobiocin , mutant , streptomyces , biology , streptomycetaceae , wild type , biochemistry , actinomycetales , genetics , gene , bacteria
Analysis of a series of 10 novobiocin non‐producing (Nov − ) mutants of Streptomyces niveus failed to detect the production of novobiocin in all pairwise combinations in cosynthesis tests. Novobiocin production was restored in nine mutants when the growth medium was supplemented with the pathway intermediates, novobiocic acid and descarbamylnovobiocin, indicating that the mutants are blocked in the synthesis of the amino coumarin moiety (Ring B). Thin layer chromatographic analysis confirmed this by showing that each mutant was able to synthesize the benzoic acid component (Ring A) but not novobiocic acid (the fusion product of Rings A and B) or descarbamylnovobiocin. A study of the novobiocin resistance characteristics of the 10 mutants showed resistance levels considerably lower than for wild type. The Nov − mutants could be classified into four phenotypic groupings (Ib + Is + , Ib + Is − , Ib − Is + and Ib − Is − ) on the basis of their ability to be induced to high level resistance (Ib) by an inducing factor produced by wild‐type mycelium and their ability to induce high level resistance (Is) in wild type.