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Thermostable dextranases: screening, detection and preliminary characterization
Author(s) -
Wynter C.V.A.,
Galea C.F.,
Cox L.M.,
Dawson M.W.,
Patel B.K.,
Hamilton S.,
Jersey J. De,
Inkerman P.A.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb00936.x
Subject(s) - thermostability , dextranase , thermophile , bacteria , sugar , agar , dextran , chemistry , isolation (microbiology) , chromatography , food science , biochemistry , microbiology and biotechnology , biology , enzyme , genetics
Screening methods have been developed for detection of micro‐organisms producing thermostable dextranases. They utilize the incorporation of Blue Dextran into agar or liquid culture media for isolation of active dextranase producers growing at temperatures above 55°C. A variety of high temperature environments in sugar factories and naturally occurring thermal water samples were excellent sources of dextranase producers. A number of aerobic and anaerobic thermophilic bacteria, isolated from these sources, were found to produce thermostable dextranases. Dextranases with the greatest thermostability were found in cultures of anaerobic bacteria grown above 65°C. Temperature optima were determined for several crude enzyme preparations, four of which exhibited temperature optima in the range 65–85°C.