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Identification of enterohaemorrhagic Escherichia coli by means of their production of enterohaemolysin
Author(s) -
Bettelheim K.A.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb00932.x
Subject(s) - vtec , escherichia coli , microbiology and biotechnology , biology , agar , identification (biology) , agar plate , bacteria , ecology , genetics , gene
With increasing interest in verocytotoxigenic Escherichia coli (VTEC) being associated with both human and animal infection, a simple system of rapidly identifying the majority of VTEC has been devised. This depends on the fact that most VTEC produce enterohaemolysin, which is rarely produced by non‐VTEC. By employing two media, traditional sheep blood agar (SBA) and washed sheep blood agar supplemented with calcium (WSBA‐Ca), enterohaemolysin‐producing strains can be easily differentiated from other E. coli enabling most VTEC types, including ones belonging to serogroups other than O157, to be isolated.