Premium
Polygalacturonase production by Kluyveromyces marxianus: effect of medium composition
Author(s) -
Schwan Rosane F.,
Rose A.H.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb04416.x
Subject(s) - fructose , sucrose , kluyveromyces marxianus , xylose , secretion , fermentation , chemistry , galactose , biochemistry , ethanol , growth medium , kluyveromyces , ammonium , yeast , food science , biology , saccharomyces cerevisiae , organic chemistry , bacteria , genetics
A strain of Kluyveromyces marxianus (CCT 3172), isolated from a cocoa fermentation in Brazil, secreted an endopolygalacturonase (PG) when grown under self‐induced anaerobic conditions; neither polymethylesterase nor pectate lyase appeared in culture filtrates. Replacing glucose in the medium with sucrose had no effect on PG secretion or ethanol production. Growth in fructose‐containing medium retarded secretion of PG and ethanol, but had no effect on growth. Growth and ethanol production in media containing galactose resembled those in fructose‐containing medium, although PG secretion was lowered. Growth and PG secretion were considerably retarded in xylose‐containing medium, and were similarly affected in media containing different concentrations of glucose. Varying the concentration of ammonium sulphate in media had no effect on growth or PG secretion.