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Pulsed‐field gel electrophoresis‐fingerprinting, genome size estimation and rrn loci number of Rhizobium galegae
Author(s) -
Huber Ingrid,
SelenskaPobell Sonja
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb04397.x
Subject(s) - pulsed field gel electrophoresis , biology , restriction enzyme , genetics , restriction fragment length polymorphism , genome , genome size , restriction fragment , gel electrophoresis , dna , polymerase chain reaction , gene , genotype
I. HUBER AND S. SELENSKA‐POBELL. 1994. The genomes of several Rhizobium galegae (of.) strains, which effectively nodulate Galega officinalis host, were analysed by pulsed‐field gel electrophoresis (PFGE). Individual PFGE‐fingerprints were obtained for every particular strain when the rarely cutting restriction endonucleases Spe I and Asn I were applied. In hybridization experiments, where a DNA fragment carrying the rrnB ribosomal RNA operon of Escherichia coli was used as a probe, the number of the resulting strain‐specific Spe I and Asn I bands was reduced to three for all of the strains studied. This suggests that in Rh. galegae (of.) there are at least three rrn loci. On the basis of the lengths of the Spe I fragments, separated by PFGE, the genome size of five Rh. galegae (of.) strains was estimated to be 5852 ± 198 kbp.